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MARIN BIOLOGIC LABORATORIES, INC.
Pre-clinical Contract Laboratory Services
Research, Assay Development, Validation and Testing Services
Contract Research Organization - CRO - GLP GMP Compliant
BIOCHEMISTRY
ELISA Test 
Enzyme Assay Protein Production,
Purification, Analysis
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BIOCHEMISTRY OVERVIEW
Marin Biologic's scientists are classically
trained biochemists with extensive experience in protein purification,
characterization and analysis. We have purified native proteins
to homogeneity isolated from cell culture and tissue as well
as recombinant proteins from bacteria and mammalian cells and
from baculovirus infected insect cells.
ELISA TEST
Frequently, we are asked to develop an
ELISA test to quantify specific proteins or small molecules
(metabolites) for a cell-based
assay. We will work with you to develop or to in-source
a commercially available ELISA test or other immunoassays
that will quantitatively detect your molecule. Our scientists
will consult with you and discuss the most efficient strategies
that can be employed. Should you need specific monoclonal antibodies
to use in your ELISA test, we can generate the hybridomas and
produce monoclonal
antibodies up to 1 gram per week.
ENZYME ASSAY
Enzyme assays have been used to monitor protein purification
and production. If your pharmaceutical is an enzyme inhibitor,
we have used enzyme assays to characterize drug potency and
measure drug concentration. Some of the enzyme assays we have
developed are used for drug stability as well as lot release.
PROTEIN PRODUCTION, PURIFICATION, ANALYSIS
Our scientists will consult with you on optimizing protein production,
protein purification and analytical analysis. If your protein
is generated via recombinant methods, we will employ the appropriate
affinity methods of purification. Should you wish a homogeneously
purified protein, we will discuss the strategies with you prior
to final purification. Of course, if you have a protein purification
strategy, we will gladly employ your purification scheme. With
many of the protein purification protocols, we will develop
a purification scheme to monitor increase in yields and specific
activity. Should you not have a method to quantitate protein
activity; our scientists will consult with you about assay
development.
The following is a partial list of techniques we perform.
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