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MARIN BIOLOGIC LABORATORIES, INC.
Pre-clinical Contract Laboratory Services
Research, Assay Development, Validation and Testing Services
Contract Research Organization - CRO - GLP GMP Compliant
CELL BIOLOGY
Cell-Based Assay 
Cell Culture Bioassay Development
Protein Expression
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CELL BIOLOGY OVERVIEW
More than 20 years experience in cell
biology and cell culture enables our staff at Marin Biologic
to employ both the scientific approach and the "art"
of cell culture to your projects. We can recommend strategies
and provide contract services for cell-based assays, tissue
culture, cell culture optimization, bioassays including stem
cells and protein expression systems for large-scale tissue
culture (including hollow fiber). Our laboratory is GLP
and GMP compliant for projects destined for FDA submission,
allowing your custom
assay development to be brought through pre-clinical
assay validation to clinical drug development.
CELL-BASED ASSAY, BIOASSAY DEVELOPMENT
Cell-based assay or bioassay development
can range from cytotoxic assays including apoptosis to cell
proliferation and metabolic assays. Cell-based assay development
can also include high throughput screening assays and other
custom bioassays used to characterize drug stability for GLP
GMP lot release, drug potency and for drug purification and
production support. Mechanisms of action, such as receptor binding,
receptor activation, cell signaling, drug internalization and
subcellular localization can be delineated in cell-based assays
following treatment with your pharmaceuticals. Bioassay development
can encompass testing of conditioned medium, cell lysates or
whole cells in a variety of formats including ELISA and immunohistochemical
methods. Our scientists can grow and differentiate stem
cells as well as provide assay
development to monitor stem cell differentiation using specific
gene expression.
CUSTOM CELL CULTURE
Custom cell culture can be performed
for your cells, whether it is primary explants, cloning and
optimizing expression of a cell line or developing the optimum
medium, to include serum-free medium. Large scale cell cultures
using bioreactors can be implemented for production of your
specific protein. Additionally purification of your protein
can be performed from either the cells themselves (eg. baculovirus)
or from the conditioned media (see Biochemistry).
PROTEIN EXPRESSION
Using mammalian or insect cell cultures,
our scientists can manufacture proteins on a 100 gram level.
Our staff can provide cell pellets, conditioned media or membrane
preparations. With our experience in classical protein purification
(see Biochemistry)
we can optimize your protein expression and production. Our
scientists are very experienced in transfection and stable cell
line generation.
The following partial list of methods is
an example of our technologies that have been performed by
our scientists.
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Receptor and Protein Mediated Transport |
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Transient and Stable Transfection |
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Electroporation |
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Subcellular Localization |
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Mechanisms of Internalization |
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