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MARIN BIOLOGIC LABORATORIES, INC.
Pre-clinical Contract Laboratory Services
Research, Assay Development, Validation and Testing Services
Contract Research Organization - CRO - GLP GMP Compliant
IMMUNOLOGY
Cell-Based Immunoassay 
Monoclonal Antibodies ELISA
Assay Development
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IMMUNOLOGY OVERVIEW
The scientists at Marin Biologic are
experienced in producing monoclonal antibodies as well as developing
and running ELISA assays and other immunological assays for
research, development and GLP and GMP compliant projects. Our
expertise in cell biology
enables us to develop, validate and perform cell-based
assays from primary tissue, such as bone marrow, spleen,
blood, as well as with tissue culture cell lines.
CELL-BASED IMMUNOASSAY
Many of our clients develop pharmaceuticals
that require cell-based immunoassays to measure an immune response.
We can test sera not only for IgG or other antibody isotype
responses but can determined whether cell activation is involved,
by using cell-based immunoassays such as the mixed lymphocyte
reaction (MLR) or natural killer cell activation. We can determine
for toxicity studies whether cytotoxic T lymphocytes have been
sensitized to your drug or whether the immune system cells have
been activated by the detection of released cytokines or the
presence of a specific cellular receptor (see Cell
Biology).
MONOCLONAL ANTIBODIES
Often cell-based immunoassays require
the pairing of two antibodies, one for capture and one for detection
(see ELISA assay below).
Since polyclonal antibody affinities change over time as the
T cells mature, an assay employing monoclonal antibodies may
result in a more reproducible assay. Additionally, affinity
purification using a monoclonal antibody will also result in
reproducible yields. Our scientists will consult with you about
producing hybridomas that generate monoclonals with the desired
characteristics. We will conjugate your hapten, or if your antigen
is a protein, we will suggest an immunization protocol. We will
develop your screening assay or implement the assay you've developed
and screen your initial cell cultures after fusion as well as
your clones. We will deliver to you conditioned media for further
screens or after consultation with you, purify the antibody
and deliver the purified monoclonal antibodies as well as the
hybridomas. If you need further characterization of the monoclonal
antibodies, our scientists will discuss with you your needs
and perform the needed analyses.
ELISA ASSAY
ELISA
assay development and validation is frequently needed for
drug purification, stability or lot release. We can develop
your ELISA assay using polyclonal or monoclonal antibodies supplied
by you or use the antibodies we develop here at Marin Biologic.
Our scientists will consult with you about your final use for
the ELISA assay and discuss various formats that can be designed
into you assay development. Should you have an ELISA assay already
developed and wish to have our scientists use it to test drug
metabolism, drug stability or lot release we will employ your
protocols.
This partial list indicates the techniques
we perform.
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