MARIN BIOLOGIC LABORATORIES, INC.
Pre-clinical Contract Laboratory Services
Research, Assay Development, Validation and Testing Services
Contract Research Organization - CRO - GLP GMP Compliant

MOLECULAR BIOLOGY

Gene Expression cDNA Library DNA Isolation Reporter Assay

MOLECULAR BIOLOGY OVERVIEW

The scientists at Marin Biologic have extensive experience in cloning and analyzing genes from both prokaryotic and eukaryotic cells. We will work with you to develop expression, production and analysis strategies and take into account your final use. With our experience in biochemistry, we can purify your protein to homogeneity using molecular biology and biochemistry approaches.

GENE EXPRESSION

Marin Biologic scientists can also express your gene of interest. We are able to produce constructs in the system of your choice. From your expression construct we can further express the protein of interest and test it in other cell-based assays. If you are working with siRNA, we have experience in regulating mammalian genes using this technology.

cDNA LIBRARY

We can create genomic or cDNA libraries for you from tissues or cell culture with expertise. Not only can we make your DNA library, we can clone your gene of interest for analysis in a customized bioassay.

DNA ISOLATION

If you already have your gene of interest subcloned we can perform the DNA isolation for you. If not, we are skilled in subcloning and can further perform your gene expression analysis or subclone your gene of interest into a mammalian expression vector. With our experience in cell culture, we can expand your cells and harvest your protein for purification (see Cell Biology and Biochemistry).

REPORTER ASSAY

Our scientists are able to perform various reporter assays with your reporter constructs. We have experience in many different reporter assays such as CAT assays, luciferase assays and various other fluorescent or chemiluminescent reporter assays. We can always create a custom reporter assay for you.

Below is a partial list of the techniques performed.

RNA Analysis

RNA DNA Isolation

	Northern Blot
	Dot Blot or Slot Blot Hybridization
	RT-PCR, Reverse-Transcriptase Polymerase Chain Reaction
	In situ Hybridization
	5', 3' RACE
	RNase Protection Assay

	Eukaryotic and Prokayrotic (Bacteria, Yeast, Plant, Baculovirus, Tissue Culture Cells, Primary Tissues)
	Total RNA
	mRNA
	Genomic and Plasmid DNA
	cDNA Purification for Microarrays
	Small and Large Scale

Reporter Assay

Gene Expression and Cloning

	CAT Assays
	Luciferase Assays
	Fluorescence Assays
	Chemiluminescence Assays
	Custom Assays

	Restriction Endonuclease Digestion and Fragment Mapping
	DNA Subcloning - High Throughput
	cDNA Library and Genomic Library
	Site-Directed Mutagenesis
	siRNA

Protein Production and Expression

Recombinant Protein Expression and Manufacture
(Baculovirus, Bacteria, Yeast)

MARIN BIOLOGIC LABORATORIES, INC. Pre-clinical Contract Laboratory Services Research, Assay Development, Validation and Testing Services Contract Research Organization - CRO - GLP GMP Compliant