Off-the-shelf assays

Using Custom Laboratory Services we can provide you with the Assays you need.  GLP studies are available as well. If you don’t find the Assay listed here that you need, then just call us to explore how we can meet your needs. Call us at 415-883-8000. It's that easy.

Antibodies

Rabbit Antiserum, 50mL
Supply 1 to 100 mg protein to be used as antigen

Monoclonal Antibody – Hybridoma Derivation

Monoclonal Antibody Production
5 L batches of conditioned medium,  5 – 20mg MAb
100mL ascites fluid, 200 – 1000mg MAb

IgG Purification, 10 ml antiserum, ascites or 100s L culture medium

F(ab’)2 Preparation
Supply 50 mg of antibody

Fab Preparation
Supply 50 mg of antibody

Fluorescein Conjugation
Supply 100 microgram-1mg of your protein and antibody.  Other fluorophores available.

Biotin Conjugation
Supply 100 microgram-1mg of your protein and antibody. 

Peroxidase Conjugation
Supply 100 microgram-1mg of your protein and antibody.  Other enzymes available.

Peptide Conjugation for immunizations and antibody purification
Supply 10 mg peptide. We can synthesize peptides for you.

Affinity Pure for specific antigen, 10 ml antiserum, ascites or culture medium    

Antibody Isotyping, determine the IgG, IgM  or IgA class of antibody

Affinity measurement,  Kd

Antibody Immunoassays

Western Blot
Supply 100 micrograms of your protein and antiserum/antibody.  Data will be an electronic image.  The blot can be scanned by digital densitometry.

Dot Blot
Supply 100 micrograms of your protein and antiserum/antibody.  Data will be an electronic image.

ELISA Development

MSD, Meso Scale Discovery
Platform for increased sensitivity, expanded range and decreased matrix effects.

Inquire
Supply 100 micrograms of your protein and antibody

RIA Radioimmunoassay
Inquire

Cellular Immunoassays

Mouse Lymphocytes
Mononuclear leukocytes cells from spleen.  Purified subsets of T or B cell and blood or lymph nodes sources are available.

Human Lymphocytes
Mononuclear leukocytes cells from blood. Purified granulocytes, monocytes or subsets of T or B cells are available.

Cell Biology

Cytoxicity/Proliferation
Human lymphocyte line.  Adherent line and murine lines available.
20 samples or dilutions/plate x 3plates/day, viable cell colorimetric readout
Metabolic, labeling and turnover assays available.

Cell Cloning
Adherent or non-adherent cell cloned by limit dilution, 5 clones delivered.

Cell Culture
100 ml suspension culture
5L suspension culture
100 ml adherent cell culture
Serum-free and larger volumes available

Flow Cytometry
Screen 20 samples with your antibody, printout of histogram with FS and SS.  Other analysis available.

Molecular Biology

RNA Preparation, 10 mg total RNAm  1mg mRNA, larger amounts on reduced scale

RNA Dot Blot

RNA Slot Blot

RT-PCR

Q-PCR

Transfection, Stable
Supply selectable transfection vector, two methods will be used

Plasmid Construction

Plasmid Preparation   

Restriction Fragment Mapping

Transfection in E. coli

E. coli Fermentation for Recombinant Protein, 5 L, multi-mg cell paste

Recombinant Protein Extraction from E. coli,

Biochemistry

Enzyme Assay
Inhibitor, kinetics, temperature dependence, pH optimum, enzyme stability available

Purity, 12 samples
SDS-PAGE with Coomassie staining

Protein Purification
Inquire affinity purification, classical purification to homogenity

Pyrogen, Endotoxin measured in the LAL Assay