MARIN BIOLOGIC LABORATORIES, INC.
Pre-clinical Contract Laboratory Services
Research, Assay Development, Validation and Testing Services
Contract Research Organization - CRO - GLP GMP Compliant

SCIENTIFIC STAFF

Commitment To Our Clients   Why Contract With Marin Biologic?   Our Staff

The staff at Marin Biologic is composed of experienced scientists encompassing the disciplines of cell biology, molecular biology, biochemistry and immunology. Marin Biologic scientists also have years of experience with projects focusing on assay development, assay validation and stability studies and in vitro toxicology.

Dr. Tania Weiss, president and CEO, was formerly a faculty member at the University of California, San Francisco and is currently an Adjunct Professor at California State University, San Francisco. She has over 20 years of experience in academic research as well as ten years of contract research experience. Marin Biologic scientists hold faculty positions at University of California, San Francisco and Department of Veterans Affairs, San Francisco. The scientists have 15 to 20 years of research and biotech industry experience and have completed their scientific training at the following: University of California, Berkeley, Davis, Santa Cruz, San Diego and San Francisco, University of Michigan, Yale University, Massachusetts Institute of Technology, University of Chicago, Arizona State University, University of Illinois, Loyola University, Illinois Institute of Technology, University of Wisconsin. The publication record for juried papers and presentations range over 20 per scientist. Many of Marin Biologic's scientists hold multiple patents.

With this level of R&D experience, the scientists at Marin Biologic have developed the capability to solve complex research problems a well as perform routine analyses. The scientists are also skilled at communicating with clients. Marin Biologic scientists realize that their clients require promptness as well as quality work in completing their projects and consequently focus their efforts to accomplish these goals. The following pages give a brief summary of the experience of our scientists.

Cell Biology	Our scientists at Marin Biologic have successfully turned the art of cell culture into a creative and productive scientific strategy for our clients. We have extensive experience culturing and developing cell culture models, including myeloid, erythroid, and lymphoid cancer cells, hemopoeitic progenitor cells, neuronal cells and explants of liver, breast, ovary, lung, skin, and brain. Our staff also has experience in recombinant protein production in insect systems (Baculovirus hosts) as well as transfection of and production in mammalian cells. Some of our methodologies include the use of stationary cultures, spinner flasks/tanks, three-dimensional cell culture, bioreactor and membrane insert technologies. Our staff is dedicated to determining and defining the appropriate microenvironment, including developing custom made media. Also, we have experience in the mechanism of drug action and resistance. Our scientists have designed DNA and nucleotide based therapeutics (oligos) and studied their mechanisms of cellular uptake. Studies include a number of cellular phenomena (a) the proliferative effects of lipids, cytokines and hormones, (b) the expression of membrane receptors and specific cellular markers, (c) receptor activation turnover and phosphorylation, and intracellular signaling (d) metabolism and secretion of cellular products and (e) DNA, RNA and protein synthesis. Mechanistic studies were performed on the release and intracellular trafficking of neurotrophic factors. We have investigated drug influences on plasma and mitochondrial membrane structure and function. We have grown and harvested multi-liter quantities of adherent and suspension cells for the isolation of specific molecules such as receptors, hormones, cytokines, and monoclonal antibodies.
Molecular Biology	Marin Biologic scientists have experience in all the general molecular biology techniques including siRNA, antisense and triple helix DNA oligos, cloning, expression, sequencing, PCR, site-directed mutagenesis, nested deletions, construction of normalized cDNA libraries and PCR, RT-PCR, RNase protection assay. We have cloned, expressed and functionally analyzed through site-directed mutations several serine proteases and novel genes in acute CNS injury using a variety of methodologies. Our scientists have developed high throughput assays to detect genome-wide single nucleotide polymorphisms. The staff has experience with the expression of cloned DNA using bacteria, yeast and baculovirus expression vectors. Additionally, the Marin Biologic staff has designed and characterized gene therapy techniques including oligonucleotide regulatory DNAs and DNA uptake methods.
Biochemistry	Our scientists have purified proteins to homogeneity, immunologically identified isoenzymes, completed structure-function characterizations and studied the biosynthesis of the following proteins: FDP aldolase, carboxypeptidase, protease E, elastase II, flavin-bound enzymes, des-leu10angiotensin I protease, small redox metalloproteins (e.g. various cytochromes), novel amino acid-derived quinone redox cofactors, human monoamine oxidases A & B, clusterin and phospholipases A2. In addition to protein purification and characterization, our scientists have experience in lipid purification and the study of lipid effects on membrane function.
Immunology	Marin Biologic scientists develop and validate immunoassays including ELISA, RIA, immunoprecipatation and Western Blot analysis. Our staff produces custom hybridomas as well as generates and purifies multi-gram quantities (up to 100g) of monoclonal antibodies using hollow fiber bioreactors, spinner and traditional stationary cultures. In addition, we perform antigen conjugation to a variety of supports, antibody purification and characterization, and antibody derivatization for use in immunoassay. Our cell biologists have experience with a variety of cellular systems as described above. More specifically, our scientists have isolated the immune cells (T, B, NK and macrophage) and used them for analysis including cell activation, mixed lymphocyte reactions, cytokine production, and cytotoxicity.
in vitro Toxicology	The scientific expertise at Marin Biologic in cell biology, biochemistry and molecular biology enables us to perform custom toxicology projects for you and your laboratory. Our scientists have studied and characterized the cytotoxic effects of numerous compounds on the normal and abnormal functions of a wide collection of eukaryotic cells. Some of these studies include apoptosis, cell proliferation, replication, gene activation, transcription, cytokine production, intracellular enzyme function, metabolite synthesis and degradation. The Marin Biologic staff can aid you in determining your drug dose range, the potential cytotoxicities in your drug dose range, the potential cytoxicities in target tissues and toxicity mechanisms.
Regulatory Assay Development Validation and Stability Testing	Our staff has experience in assay development, validation and stability testing for biologics which have led to numerous successful IND filings and product marketing applications in the US and Europe. Rapid screening assays have been developed using different technologies, which enhance accelerated stability studies.