PCR-qPCR

We have extensive expertise in the creation, refinement, and validation of molecular assays utilizing PCR or Quantitative-PCR (as well as RT-qPCR), providing top-tier molecular services to meet our clients’ testing requirements. Our services include:

  • Unmatched quality
  • Rigorous quality control procedures
  • Prompt timelines
  • Cost-effective solutions
  • Exceptional communication
  • Multitarget quantitation using FAM, VIC, Cy5, TR, JOE, SYBR Green and ROX detection systems.

MarinBio assists you throughout the drug development process, including IND/BLA applications, commercialization, and pharmacokinetics (PK). We engage directly with the FDA and work closely with our clients to achieve their goals.

 

Quantitative polymerase chain reaction (qPCR) has become an indispensable tool in drug discovery, offering unparalleled sensitivity, specificity, and versatility across various stages of the pharmaceutical development process. This powerful technique allows researchers to quantify gene expression, detect genetic variations, and analyze biomarkers with high precision, making it invaluable in the quest for new therapeutic agents and personalized medicine approaches. In drug discovery, qPCR finds applications in several key areas:

  • Target identification and validation
  • Lead optimization
  • Biomarker discovery
  • Pharmacogenomics
  • Toxicology studies
  • Drug metabolism studies
  • Efficacy testing

Need Help or Have Questions on PCR or qPCR Service?

Contact us to discuss the specifics of your project. Our team of experienced scientists is ready to assist you with tailored solutions to meet your unique needs. Whether you need help with assay development, FDA IND, stability study,  lot release approval, or other scientific endeavors, we are here to support you.
Contact us to learn more about our cell based assay services

We will initiate your project as soon as the proposal is approved and signed by you. Our streamlined process ensures a quick start, minimizing delays and maximizing efficiency. Fill out the form below to email us or click our live chat option to speak with a MarinBio Scientist and get your project underway.

Yes, we have over 30 years of expertise in developing and validating cell based assays for FDA IND, lot release or stability study approval. Our team can also liaise directly with the FDA on your behalf, ensuring a smooth and compliant process. Please contact us to learn more about how we can assist you with your assay development and FDA approval needs.

MarinBio scientists are experienced and creative in designing, developing, validating assays. Some of the assays we perform are listed below, but there are many more that we created, developed and validated. Give us call so that we may help you.

Need help or have questions on cell based assays?

Give us a call at 415-883-8000

Gene Expression in Drug Treated Cells by qPCR

For gene expression in client studies, MarinBio cultures cells, treats these cells with appropriate concentrations of a client’s drugs or control agents, isolates the cellular messenger RNA (mRNA) and using multiplex RT-qPCR detects target gene expression.

Apart from gene expression, MarinBio professionals have extensive experience using the Abcam FirePlex system for analyzing miRNAs directly from small amounts of tissues, cultured cells, other biofluids.

The assay consists of (a) capture of selected miRNAs on encoded hydrogel particles, (b) ligation of specific adapters to each bound miRNA, (c) target amplification using a single, universal primer and (d) recapture of amplified targets onto the hydrogel particles. Assay readout is performed using a flow cytometer. The use of the state-of-the-art MarinBio Attune NxT Cytometer Flow Cytometer enables automated profiling of up to 65 or more target miRNAs of choice simultaneously across tens to hundreds or more samples.

Mutation Identification by PCR

PCR for mutation identification using gel electrophoresis involves extracting DNA from the

sample, amplifying the target region with primers via PCR, and preparing an agarose gel.

The PCR products are loaded into the gel, and an electric current is applied to separate

the fragments by size. After staining, the gel is visualized under UV light to compare

the sample’s band pattern against a control. Differences in band size or restriction patterns

indicate the presence of mutations such as insertions, deletions, or point mutations.

KEY DIFFERENCES BETWEEN QPCR, RT-PCR AND RT-QPCR:

  • qPCR focuses on the quantification of DNA and does not involve reverse transcription.
  • RT-PCR focuses on converting RNA to cDNA and then amplifying it, but does not provide quantitative data.
  • RT-qPCR combines both processes, allowing for the quantification of RNA by first converting it to cDNA and then performing quantitative PCR.

Case Study- Time-Dependent Gene Expression Modulation via siRNA: A QRT-PCR Analysis

The figure below demonstrates the dynamic regulation of gene expression in human cells following transfection with siRNA specific for Gene A. The QRT-PCR analysis reveals a time-dependent suppression of Gene A expression, accompanied by the activation of Gene B expression. This indicates that the siRNA effectively targets Gene A, leading to its decreased expression, while concurrently triggering a compensatory increase in Gene B expression over the observed time points post-transfection.

 

                                                   RT-qPCR Analysis                                                 qPCR 

                                                                  

Contact Us about Our Cell Based Assays Services

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