Binding Assays

  • Radioligand Binding Assays

           Use radiolabeled ligands to determine the affinity and number of receptors and are one of the most sensitive types of assays.

  • Competition Binding Assays

            These are used to measure the ability of unlabeled ligands to compete with radiolabeled ligands for receptor binding.

  • Fluorescence Polarization (FP) Assays

           These assays use changes in fluorescence polarization to detect ligand binding to receptors.

  • FRET (Fluorescence Resonance Energy Transfer) Assays

           FRET assays detect binding through energy transfer between fluorescently labeled ligand and receptor.

  • ELISA-Based Binding Assays

           ELISA techniques can be adapted to quantify receptor-ligand interactions, using immobilized cells or membranes.

           Analyze binding events on cell surface receptors using fluorescently labeled ligands.

Reporter Assays for Receptor-Drug Interactions

          These reporter assays utilize luciferase enzymes to produce luminescence in response to receptor activation or inhibition by a drug.

          Beta-galactosidase is used to generate a colorimetric or fluorescent signal upon receptor activation.

          GFP is employed to visualize and quantify receptor activity based on fluorescence.

         These assays measure secreted alkaline phosphatase activity in the culture medium as a readout of receptor activity.

          Measure cyclic AMP levels as a downstream readout of receptor activation using various reporter systems.

  • (CRE) cAMP Response Element-luciferase Assays

          Measure cAMP response element (CRE) activation via luciferase expression as a readout of GPCR (G Protein-Coupled Receptor) activity.

        These assays combine firefly and Renilla luciferase to measure receptor activation and normalization controls simultaneously.

        Use NFAT-responsive elements to drive reporter gene expression, indicating receptor activity.

  • NF-kB (Nuclear Factor kappa-light-chain-enhancer of activated B cells) Reporter Assays

          Measure activation of NF-kB pathways through reporter gene expression.

  • Calcium Mobilization Assays

         Detect changes in intracellular calcium levels using fluorescent or luminescent calcium indicators in response to receptor activation.

       Monitor activation of Gq or Gi protein-coupled receptors using specific reporter constructs.

Need Help or Have Questions on Cell-Based Assays?

Contact us to discuss the specifics of your project. Our team of experienced scientists is ready to assist you with tailored solutions to meet your unique needs. Whether you need help with assay development, FDA IND, stability study,  lot release approval, or other scientific endeavors, we are here to support you.
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We will initiate your project as soon as the proposal is approved and signed by you. Our streamlined process ensures a quick start, minimizing delays and maximizing efficiency. Fill out the form below to email us or click our live chat option to speak with a MarinBio Scientist and get your project underway.

Yes, we have over 30 years of expertise in developing and validating cell based assays for FDA IND, lot release or stability study approval. Our team can also liaise directly with the FDA on your behalf, ensuring a smooth and compliant process. Please contact us to learn more about how we can assist you with your assay development and FDA approval needs.

MarinBio scientists are experienced and creative in designing, developing, validating assays. Some of the assays we perform are listed below, but there are many more that we created, developed and validated. Give us call so that we may help you.

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