• Binding Affinity Determination

    Posted on Nov 15, 2018

    The strength of antibody binding to its ligand is assessed by radioimmune assay (RIA), ELISA, or binding in a column support format. Dissociation is performed by increasing denaturating conditions, or by competition with a related ligand. The dissociation constant, Kd, is determined by a Scatchard plot.

    « Back to Glossary Index