Dot Blot and Slot Blot
Posted on Dec 27, 2018
Specific RNA species in an unfractionated preparation can be measured by immobilizing a sample in a spot (Dot Blot) or in a manifold slot (Slot Blot). Detection is by a labeled DNA probe that hybridizes to the immobilized RNA. For the Dot Blot, quantitation is usually visual whereas the Slot Blot format is more easily quantitated by scanning with a densitometer.