ELISpot or enzyme-linked immunosorbent spot, allows for the detection of cells that secrete cytokines or antibodies. ELISpot is able to detect a single cell that secretes a protein of interest making it one of the most sensitive cellular assays. Cytokine-specific antibodies are placed onto an ELISpot plate. Cells of interest are then added. Cells are activated and produce cytokine which binds to the antibodies, which are detected by an enzyme-substrate method. A colored product produces a spot that is counted through a microscope

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