To screen a library of thousands of compounds, a high throughput screening assay will facilitate the identification of potential “hits” in less time than a typical assay. A high throughput assay typically is not complicated, must be very reproducible and have a high signal-to-noise ratio in order to minimize false positives. The method could be a binding assay to cells or to molecular target using ELISA, a bioassay or using a subcellular fraction, or an enzyme activity assay, Hits are then validated by more specific activity assays.

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