• Library Screening and Gene Cloning

    Posted on Dec 27, 2018

    A DNA library is screened to determine the presence of a gene and to clone out either a full-length or smaller version of the gene. There are many strategies for cloning from a library. Typically, the library is introduced into bacteria, the bacteria are plated under cloning conditions, replicate plates of the bacterial colonies are made, and the clones are probed for the existence of the desired gene either by hybridization or through the expression of the specific protein. For a positive clone, the DNA is extracted from original plate, recloned and purified.

    « Back to Glossary Index