• Peptides and Haptens

    Posted on Dec 27, 2018

    Peptides or haptens can be conjugated with various carrier proteins for use as immunogens in animals such as mice, rabbits and goats. Conjugates are also useful for affinity purification of antibodies or other binding proteins. Keyhole limpet haemocyanin (KLH) is routinely used as a carrier protein, with conjugation of the peptide to the carrier protein typically through an N-terminal or C-terminal amino acid. Other carrier proteins (e.g., ovalbumin, tetanus toxoid, diphtheria toxoid, tuberculin PPD) and conjugation procedures (e.g. glutaraldehyde, bis-diazotised tolidine (BDT), carbodiimide) are available upon request.

    Peptides or haptens can also be conjugated to various fluorochromes or to biotin, through covalent attachment to lysines, thiols or carbohydrates and subsequently detected with either avidin or streptavidin. Peptides can also be radiolabeled. These conjugates can be used for ELISA or RIA, or for analysis of receptor binding and cellular uptake.

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