Purified, full-length RNA is required for cDNA cloning. It is also used in some methods for analyzing gene expression and for microarrays utilizing cDNA. RNA is prepared from bacteria, tissue culture cells, tissues and plants. Preparations can be purified messenger (mRNA) or total RNA that includes mRNA, ribosomal (rRNA) and transfer RNA (tRNA). The scale is from 1 microgram to1 milligram.

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