DNA OR RNA AMPLIFICATION BY PCR OR RT-PCR
Posted on Feb 13, 2020
MarinBio has special expertise in the Polymerase Chain Reaction (PCR) as extremely versatile techniques for copying DNA or RNA molecules. PCR enables a specific nucleic acid sequence to be copied or modified, where a single DNA or RNA molecule is amplified to a billion cloned identical molecules in less than two hours by PCR via thermal cycling. We also have experience with isothermal PCR gene amplification which is enzymatically driven at room temperature. DNA PCR determines whether a particular DNA fragment is found in a cDNA library. At MarinBio, DNA PCR is also successfully employed, for example, to introduce specific restriction enzyme sites to the ends of DNA molecules or to mutate particular DNA bases in site-directed mutagenesis. Reverse Transcriptase-PCR (RT-PCR) amplifies RNA. Quantitative PCR (QPCR) of DNA and Quantitative RT-PCR of RNA enables quantitative measurements for determining precise amounts of DNA or RNA molecules, respectively.