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Binding Antibody Neutralization Assays

In 2024, MarinBio successfully passed the FDA inspection with no 483s and no observations.

Binding Assays

  • Radioligand Binding Assays:
    Use radiolabeled ligands to determine the affinity and number of receptors. These are one of the most sensitive types of assays.
  • Competition Binding Assays:
    These assays measure the ability of unlabeled ligands to compete with radiolabeled ligands for receptor binding.
  • Fluorescence Polarization (FP) Assays:
    These assays use changes in fluorescence polarization to detect ligand binding to receptors.
  • FRET (Fluorescence Resonance Energy Transfer) Assays:
    FRET assays detect binding through energy transfer between fluorescently labeled ligand and receptor.
  • ELISA-Based Binding Assays:
    ELISA techniques can be adapted to quantify receptor-ligand interactions using immobilized cells or membranes.
  • Flow Cytometry-Based Binding Assays:
    These assays analyze binding events on cell surface receptors using fluorescently labeled ligands.

Reporter Assays for Receptor-Drug Interactions

  • Luciferase Reporter Assays:
    These reporter assays utilize luciferase enzymes to produce luminescence in response to receptor activation or inhibition by a drug.
  • Beta-Galactosidase Reporter Assays:
    Beta-galactosidase is used to generate a colorimetric or fluorescent signal upon receptor activation.
  • GFP (Green Fluorescent Protein) Reporter Assays:
    GFP is employed to visualize and quantify receptor activity based on fluorescence.
  • SEAP (Secreted Alkaline Phosphatase) Reporter Assays:
    These assays measure secreted alkaline phosphatase activity in the culture medium as a readout of receptor activity.
  • cAMP Reporter Assays:
    Measure cyclic AMP levels as a downstream readout of receptor activation using various reporter systems.
  • (CRE) cAMP Response Element-luciferase Assays:
    Measure cAMP response element (CRE) activation via luciferase expression as a readout of GPCR (G Protein-Coupled Receptor) activity.
  • Dual-Luciferase Reporter Assays:
    These assays combine firefly and Renilla luciferase to measure receptor activation and normalization controls simultaneously.
  • NFAT (Nuclear Factor of Activated T-cells) Reporter Assays:
    Use NFAT-responsive elements to drive reporter gene expression, indicating receptor activity.
  • NF-kB (Nuclear Factor kappa-light-chain-enhancer of activated B cells) Reporter Assays:
    Measure activation of NF-kB pathways through reporter gene expression.
  • Calcium Mobilization Assays:
    Detect changes in intracellular calcium levels using fluorescent or luminescent calcium indicators in response to receptor activation.
  • Gq and Gi Protein Pathway Reporter Assays:
    Monitor activation of Gq or Gi protein-coupled receptors using specific reporter constructs.

Questions about Our Cell Based Assay Services?​

Contact us to discuss the specifics of your project. Our team of experienced scientists is ready to assist you with tailored solutions to meet your unique needs. Whether you need help with assay development, FDA IND, stability study,  lot release approval, or other scientific endeavors, we are here to support you.
Contact us to learn more about our cell based assay services

We will initiate your project as soon as the proposal is approved and signed by you. Our streamlined process ensures a quick start, minimizing delays and maximizing efficiency. Fill out the form below to email us or click our live chat option to speak with a MarinBio Scientist and get your project underway.

Yes, we have over 30 years of expertise in developing and validating cell based assays for FDA IND, lot release or stability study approval. Our team can also liaise directly with the FDA on your behalf, ensuring a smooth and compliant process. Please contact us to learn more about how we can assist you with your assay development and FDA approval needs.

MarinBio scientists are experienced and creative in designing, developing, validating assays. Some of the assays we perform are listed below, but there are many more that we created, developed and validated. Give us call so that we may help you.

MarinBio scientists conducting targeted protein degradation assays in advanced laboratory

Need help or have questions on cell based assays?

Give us a call at 415-883-8000

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