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Kinase/Phosphorylation Assays

In 2024, MarinBio successfully passed the FDA inspection with no 483s and no observations.

Cell Based Kinase/Phosphorylation Assays

  • Cellular Phosphorylation Assay:
    This assay quantifies the phosphorylation status of a protein in cells. It can be performed with cells expressing either endogenous or overexpressed kinases. The phosphorylation is detected using phospho-site-specific antibodies via ELISA or AlphaLISA.
  • Cell-Deliverable Peptide Substrate Assay:
    Uses cell-penetrating peptides designed with kinase substrate sequences. These peptides are taken up by cells and phosphorylated by active kinases. Phosphorylation is then measured using ELISA or other detection methods.
Ubiquitin-proteasome system in cell-based assays for drug discovery

Cell Based Reactive Oxygen Species (ROS) Assays

  • Fluorescence-Based Assays:
    H₂DCF-DA (2′,7′-Dichlorodihydrofluorescein Diacetate) and DHE (Dihydroethidium) assays to detect superoxide radicals in cells.
  • CellROX Reagents:
    Used for detecting ROS in live cells using imaging, flow cytometry, or microplate analysis.
  • MitoSOX Red:
    Used to detect superoxide production in mitochondria.
  • CM-H₂DCFDA (Chloromethyl-H₂DCFDA):
    Used for detecting ROS in live cells, particularly hydrogen peroxide, superoxide, and hydroxyl radicals.
  • Chemiluminescence-Based Assays:
    Used for detecting various ROS, including hydrogen peroxide, superoxide, and hydroxyl radicals in biological samples.
  • Spectrophotometric Assays:
    FOX (Ferric-Xylenol Orange) Assay.
  • Genetically Encoded Sensors:
    roGFP (Redox-Sensitive Green Fluorescent Protein).

Cell Based Nitric Oxide Synthase (NOS) Assays

  • Intracellular Nitric Oxide Assay (Fluorometric):
    Utilizes a nitric oxide-specific fluorescent probe that is highly photostable and has a wide pH stability range.
  • Homogeneous Fluorescent Cell-Based Assay:
    Involves the use of ionomycin to stimulate NO production, which is then detected using a fluorescent probe.
  • Nitric Oxide (NO) Assay Kit (Colorimetric):
    Measures total NO by quantifying nitrite and nitrate, the stable end products of NO oxidation.
  • In Vitro Nitric Oxide (NO) Assays (Colorimetric and Fluorometric):
    These assays measure NO indirectly by detecting its breakdown products (nitrite and nitrate) in various biological samples.

Need Help or Have Questions on Cell-Based Assays?

Contact us to discuss the specifics of your project. Our team of experienced scientists is ready to assist you with tailored solutions to meet your unique needs. Whether you need help with assay development, FDA IND, stability study,  lot release approval, or other scientific endeavors, we are here to support you.
Contact us to learn more about our cell based assay services

We will initiate your project as soon as the proposal is approved and signed by you. Our streamlined process ensures a quick start, minimizing delays and maximizing efficiency. Fill out the form below to email us or click our live chat option to speak with a MarinBio Scientist and get your project underway.

Yes, we have over 30 years of expertise in developing and validating cell based assays for FDA IND, lot release or stability study approval. Our team can also liaise directly with the FDA on your behalf, ensuring a smooth and compliant process. Please contact us to learn more about how we can assist you with your assay development and FDA approval needs.

MarinBio scientists are experienced and creative in designing, developing, validating assays. Some of the assays we perform are listed below, but there are many more that we created, developed and validated. Give us call so that we may help you.

MarinBio scientists conducting targeted protein degradation assays in advanced laboratory

Need Help or Have Questions on Cell-Based Assays?

Give us a call at 415-883-8000

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