February 25, 2026
Need T-cell assays?
Assess activation, cytotoxicity, proliferation, and flow cytometry, ELISA, and MLR assays.
Assess immune cell activity?
Measure dendritic inflammasome activation, NK tumor killing, ADCC, and flow cytometry.
Evaluate macrophage and neutrophil functions?
Measure cytokines, phagocytosis, oxidative burst, and degranulation.
Mixed Lymphocyte Reaction is a type of T cell proliferation assay.
Activation of T cells through the T Cell Receptor (TCR) results in cellular changes.
CD69, CD25 up-regulation by flow cytometry.
ELISAs for cytokine / chemokine expression.
Phospho-specific western blots to evaluate kinase / phosphatase activity.
A T cell cytotoxicity assay measures the ability of T cells to kill target cells, indicating immune response efficacy. Cytotoxic T cells, also known as CD8+ T cells, are responsible for killing target cells.
Infection and cancer can lead to cellular exhaustion. Inability of T cells to respond to stimuli is evaluated. Cytokine response assays, Cytotoxicity assays, Flow cytometry assays.
The effect of immunomodulatory agents on T cell function is measured, particularly in the presence of regulatory T cells (Tregs).
The expression levels of inhibitory checkpoint molecules (e.g., PD-1, CTLA-4) on T cells is measured using flow cytometry, ELISA, and reporter assays.
Memory T cells can be generated in humans by a number of antigenic stimuli including tumor antigens, infection and vaccines. Recall of memory T cells in human PBMC are measured by ELISPOT and flow cytometry.
Different T cell populations are identified and quantified using antibodies against specific surface markers.
The production of cytokines within T cells after stimulation is measured.
This assay measures the proliferation of T cells in response to stimuli such as antigens or mitogens.
Inflammasome assays are used to evaluate the activation of inflammasomes within dendritic cells.
Co-cultures of PBMCs or DCs with allogeneic T cells and/or drugs are evaluated for proliferation to ascertain activation.
PBMCs synthesize and secrete specific cytokine by in response to various stimuli which are measured by FLOW or ELISA.
Human PBMC derived NK cells are cultured with tumor cell lines and evaluated for killing activity by flow cytometry.
Tumor targeting antibodies enhance killing by NK cells.
Activated NK cells produce high levels of cytokines such as IFN-γ.
NK cells up and down regulate a number of cell surface receptors such as 4-1BB after activation.
Flow cytometry is used to analyze the expression of surface and intracellular markers on B cells.
ELISA is used to quantify the amount of specific antibodies produced and secreted by B cells in response to an antigen.
This assay measures the proliferation of B cells in response to stimuli such as antigens or mitogens.
This assay assesses the activation status of B cells by measuring the expression of markers (e.g., CD69, CD86) and cytokine production.
This assay measures the production of cytokines by B cells in response to stimulation.
This assay studies the differentiation of B cells into plasma cells or memory B cells.
The production of cytokines by macrophages is measured using ELISA or multiplex bead-based flow cytometry assays.
Flow cytometry is employed to analyze the expression of surface markers indicative of macrophage activation and polarization (e.g., CD80, CD86 for M1; CD206, CD163 for M2).
Macrophages are effective at killing tumor cells through phagocytosis after recognition of cellular damage or binding to antibodies (antibody mediated cellular phagocytosis, ADCP) which can be measured in vitro by flow cytometry.
These assays differentiate macrophages into M1 (pro-inflammatory) or M2 (anti-inflammatory) phenotypes using specific cytokines like IFN-γ and IL-4 and it is quantified using flow cytometry.
This assay measures the production of reactive oxygen species (ROS) by neutrophils in response to stimuli.
The shedding of CD62L (L-selectin) from the surface of neutrophils into the supernatant media is measured as an indicator of activation.
The release of granule contents from neutrophils, such as myeloperoxidase or elastase, both components of Neutrophil Extracellular Traps (NETs) are measured using custom ELISAs.
The chemotactic ability of cells, which is their directed movement in response to chemical stimuli, is evaluated by microscopy.
Contact us to discuss the specifics of your project. Our team of experienced scientists is ready to assist you with tailored solutions to meet your unique needs. Whether you need help with assay development, FDA IND, stability study, lot release approval, or other scientific endeavors, we are here to support you.
Contact us to learn more about our cell based assay services
We will initiate your project as soon as the proposal is approved and signed by you. Our streamlined process ensures a quick start, minimizing delays and maximizing efficiency. Fill out the form below to email us or click our live chat option to speak with a MarinBio Scientist and get your project underway.
Yes, we have over 30 years of expertise in developing and validating cell based assays for FDA IND, lot release or stability study approval. Our team can also liaise directly with the FDA on your behalf, ensuring a smooth and compliant process. Please contact us to learn more about how we can assist you with your assay development and FDA approval needs.
MarinBio scientists are experienced and creative in designing, developing, validating assays. Some of the assays we perform are listed below, but there are many more that we created, developed and validated. Give us call so that we may help you.
Give us a call at 415-883-8000
Our team of immunologists have both in depth scientific background and extensive experience in drug discovery and development. We offer customized cellular immunoassay services for IND/BLA applications and commercialization. Our cellular immunoassay services offer:
We maintain direct interactions with the FDA, both independently and through our clients.
Cellular immunobioassays measure immune cell responses to stimuli, offering insights into immune system behavior. These techniques involve exposing isolated immune cells such as T cells, B cells, or macrophages, to activating agents and quantifying their responses using methods like flow cytometry or ELISA to elucidate proliferation, cytotoxicity, cytokine production and surface marker expression. These assays help identify immunomodulation targets, understand disease mechanisms, and develop treatments for immune-related conditions.
For More Information about our Cellular Immunoassay services, please use the following informational form