Molecular Biology and Microbiology

When MarinBio clients require designer protein product production, purification and characterization from transfection producing transient or stable cell lines, our staff PhD scientists have many years of experience and successfully perform these client projects. Our niche is to take cross scientific field projects in molecular biology and biochemistry, biophysical chemistry, genetics, microbiology and cell biology in bringing our breadth of expertise to successfully complete client projects, for example, as follows:

• Recombinant protein production in cultured cells –bacterial/plasmid vectored cells, –insect  cells/Baculovirus vectored, –yeast cells, –mammalian cells

• Inducible cell or viral vectored gene promotors and specific cell signaling factors for high level protein expression

• Protein size, charge, conjugation, stability; Western blots for proteins and Eastern blots for post-translational modifications

• Chromatography,  electrophoresis, charge variant analyses
  Characterization: Protein size, charge, pI (Isoelectric point, pH where net charge on protein is zero), stability, Eastern blots for post-translational modifications

• Western blots, ELISAs, proteomics, drug binding protein assays
• Enzyme assays, protein assays, reactive oxygen species assays
• Ligand binding, affinity, inhibition, metabolic and radioisotope assays
• Native protein purifications from plasma, cells, tissue extracts