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Transfection

In 2024, MarinBio successfully passed the FDA inspection with no 483s and no observations and in 2025 passed the EU inspection

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Transfection is a cornerstone of our expertise at MarinBio

  • Extensive experience optimizing transfection protocols and reagents for various cell types and applications.
  • Design and implement efficient transfection strategies for both small-scale experiments and large-scale protein production.
  • Develop cell-based assays, creating stable cell lines and reporter systems for drug screening.
  • Customized assays for meaningful biological readouts tailored to client targets.
  • Decades of expertise in evaluating and optimizing new transfection reagents.
  • Rigorous testing of novel reagents across diverse cell types and payloads.
  • Accelerate your research and drug discovery with our transfection expertise. Contact us to support your specific needs.

Transfection Services We Offer

MarinBio's state-of-the-art laboratory equipment for transfection experiments

Chemical Transfection

  1. Calcium Phosphate Transfection: DNA is mixed with calcium phosphate to form a precipitate that is taken up by cells.
  2. Cationic Lipid Transfection (Lipofection): Uses lipid-based reagents to form liposomes that encapsulate the DNA and facilitate its entry into cells.
  3. Cationic Polymer Transfection: Involves the use of polymers like polyethyleneimine (PEI) to form complexes with DNA that can enter cells.
  4. DEAE-Dextran Transfection: Uses the cationic polymer DEAE-dextran to form complexes with DNA, facilitating its uptake by endocytosis.
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Physical Transfection

  1. Electroporation: Cells and DNA are exposed to an electric field that creates temporary pores in the cell membrane through which DNA can enter.
 

Biological Method

  1. Viral Transfection (Transduction): Includes Lentiviral Vectors, Adenoviral Vectors, Adeno-Associated Viral (AAV) Vectors, and Retroviral Vectors.
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Scientist performing transfection experiment using electroporation and viral transfection methods in a laboratory setting.
Nanoparticle transfection laboratory setup with modern equipment including well plates, test tubes, pipettes, and DNA/RNA encapsulation tools in a high-tech research environment.

Nanoparticle Method

  1. Types of Nanoparticles:

    • Lipid nanoparticles (LNPs)
    • Polymeric nanoparticles (e.g., polyethyleneimine, PEI)
    • Inorganic nanoparticles (e.g., gold, magnetic nanoparticles)
    • Dendrimers

    Nanoparticles encapsulate DNA/RNA and facilitate cellular uptake through lipid fusion with the cell membrane, endocytosis, or direct translocation.

Protein Transfection/Transduction

  1. Types of Methods:
    • Protein Transduction Domains (PTDs) or Cell-Penetrating Peptides (CPPs)
    • Lipid-based delivery systems
    • Nanoparticle-based delivery systems
    • Electroporation
    • Hydrogels
    • Viral transduction
Laboratory setup featuring advanced scientific equipment for protein transfection and transduction methods, including lipid-based and nanoparticle-based delivery systems, pipettes, and well plates.

Need Help or Have Questions on Transfection?

Contact us to discuss the specifics of your project. Our team of experienced scientists is ready to assist you with tailored solutions to meet your unique needs. Whether you need help with assay development, FDA IND, stability study,  lot release approval, or other scientific endeavors, we are here to support you.
Contact us to learn more about our cell based assay services

We will initiate your project as soon as the proposal is approved and signed by you. Our streamlined process ensures a quick start, minimizing delays and maximizing efficiency. Fill out the form below to email us or click our live chat option to speak with a MarinBio Scientist and get your project underway.

Yes, we have over 30 years of expertise in developing and validating cell based assays for FDA IND, lot release or stability study approval. Our team can also liaise directly with the FDA on your behalf, ensuring a smooth and compliant process. Please contact us to learn more about how we can assist you with your assay development and FDA approval needs.

MarinBio scientists are experienced and creative in designing, developing, validating assays. Some of the assays we perform are listed below, but there are many more that we created, developed and validated. Give us call so that we may help you.

MarinBio scientists conducting targeted protein degradation assays in advanced laboratory

Need Help or Have Questions on Transfection?

Give us a call at 415-883-8000

With 30 years of expertise, we specialize in transfection for:

  • Method development
  • Gene and protein expression
  • Cell-based assays

Our services include:

  • Unmatched quality
  • Prompt timelines
  • Cost-effective solutions
  • Exceptional communication

MarinBio assists you throughout the drug development process, including IND/BLA applications, commercialization, and pharmacokinetics (PK). We engage directly with the FDA and work closely with our clients to achieve their goals.

MarinBio's state-of-the-art laboratory equipment for transfection experiments
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Transfection is a laboratory technique used to introduce nucleic acids (such as DNA or RNA) or proteins or other biomolecules into eukaryotic cells. This process enables researchers to study gene function and protein expression, create genetically modified cells, and develop cell-based assays. Transfection can be accomplished using various methods, including chemical, physical, and viral-based techniques, depending on the specific requirements of the experiment and the type of cells being used.

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