PCR is a very sensitive method for detecting specific DNA and RNA segments. It is used to determine whether a gene is present in the genome or to detect the level of gene expression in the case of drug action or genetic manipulation.
Classical PCR greatly amplifies a specific sequence of DNA for analysis or molecular cloning. qPCR starts with mRNA or total RNA, makes a cDNA complimentary strand using reverse transcriptase, and then amplifies the product. The amount of cDNA, and therefore mRNA, present in a sample can be quantitatively determined based on a standard of known….
A library is an amplified collection of DNA representing the total DNA of an organism (genomic library) or of DNA complementary to a preparation of mRNA (cDNA library). Either library can be probed to pull out a specific gene of interest. cDNA libraries are used to clone the expressed version of a gene comprised of….
DNA complementary (cDNA) to mRNA is prepared for preparing microarrays for screening in different cell sources. For screening, the total RNA is extracted from tissue or cells and is hybridized to the cDNA immobilized onto a solid support. A detection molecule is applied and is visualized.